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COVID-19: Nigeria makes history as NCDC validates viral RNA extraction

The Nigeria Centre for Disease Control (NCDC ) has successfully validated the first phase of the viral Ribonucleic Acid (RNA) extraction, which should enable local production of cheap testing kits for COVID-19.

This makes Nigeria the first country in Africa to do so.
The validation took place in the laboratory of the National Biotechnology Development Agency (NABDA) in Abuja.

The Director-General of NABDA, Prof. Alex Akpa, said after the exercise that the success of the validation was important not only to Nigeria but Africa as a whole.

“The success of this validation shall enable us to massively produce test kits so that more people would be tested for COVID-19 , not only in Nigeria but in Africa.“The immediate aim is to produce reagents for real time Polymerase Chain Reaction (PCR), and remember, recently the lack of reagents stalled work in Kano and molecular diagnosis could no longer take place,’’ Akpa said.

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He also recalled that the absence of the same reagent equally stalled COVID-19 diagnosis in Lagos for many days.“This project is, therefore, designed to enable not only Nigeria but the whole of Africa to put the issue of shortage of reagents behind,’’ the director-general said.

He disclosed that that the project was a Pan-African project whose partners include Ethiopia, NCDC and the University of Sheffield, U.K., among others with funding to come from African Development Bank.Dr Ndodo Nnaemeka, Chief Molecular Bioengineer, National Reference Laboratory of NCDC, said he was mandated by the NCDC Director-General, Dr. Chikwe Ihekweazu, to “launch this wonderful project.’’

According to Nnaemeka, the project is designed to solve the problem of RNA extraction kit which had become a global issue.He said there were serious concerns for the need to have more extraction kits in-country, and that there was growing demand worldwide for extraction kits.

“The western world prioritises their own interest first by making sure that they meet their local needs before exporting to other countries, so there was really need for it,’’ he said.

The NCDC chief molecular bioengineer said the first phase of the evaluation results he carried out with the NABDA scientific team was “awesome and successful.’’

“The kits compete favourably well with other international kits we are using, in fact, it scored highly in purity and in quantity of extraction and we are thinking of scaling production,’’ Nnaemeka said.

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He clarified that to be able to conduct test, the viral information which comes as the RNA has to first be extracted and without this extraction, no test can be conducted.

“What will tell you that the virus is there is contained in the RNA, so RNA is required for you to be able to detect COVID-19,’’ he said.

He added that as scientists, they would soon carry out the second phase of the evaluation which would involve real sample of COVID-19.
Similarly, Dr. Rose Gidado, a scientist at NABDA and Country Coordinator, Open Forum on Agricultural Biotechnology (OFAB), said the exercise was historic.

She said the validated RNAswift Test Kit for COVID-19 was developed by Dr Alison Nwokeoji, a Nigerian scientist at the University of Sheffield, U.K.
She corroborated that the African Development Bank was taking over the manufacture of the test kits so as to expand Nigeria’s testing capacity with emphasis on farmers’ health.

Gidado also disclosed that the new test kit could test more than 20,000 people per day.
“If we set up more testing centres, we can easily do 50,000 with automation,’’ the scientist said.

She added that the project was a partnership involving NABDA, NCDC, Ethiopia, NVRI Vom, NIMR and the Ministries of Science and Technology, Health and Agriculture.

NAN reports that the evaluation and validation of the RNAswift Test Kit for COVID-19 was carried out in the immunology laboratory of the NABDA.
It was done in the presence of a donor, Mr Bolaji Akinboro of Cellulant Nig. Ltd, and Mr Richard Mbaram, the Technical Adviser to the Minister of Agriculture and Rural Development, Alhaji Sabo Nanono, among others. (NAN)

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